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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXP4 Polyclonal antibody

FOXP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29311-1-AP

Synonyms

FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXP4 Polyclonal antibody (29311-1-AP)

    WB analysis using 29311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29311-1-AP (FOXP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using FOXP4 Polyclonal antibody (29311-1-AP)

    IHC staining of mouse ovary using 29311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using FOXP4 Polyclonal antibody (29311-1-AP)

    IHC staining of mouse ovary using 29311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using FOXP4 Polyclonal antibody (29311-1-AP)

    IHC staining of mouse ovary using 29311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inCaco-2 cells, PC-3 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29311-1-AP targets FOXP4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP4 fusion protein Ag29953 相同性解析による交差性が予測される生物種
Full Name forkhead box P4
Calculated molecular weight 678 aa, 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC052803
Gene symbol FOXP4
Gene ID (NCBI) 116113
RRIDAB_2918279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.

Protocols

Product Specific Protocols
WB protocol for FOXP4 antibody 29311-1-APDownload protocol
IHC protocol for FOXP4 antibody 29311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols