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Tested Applications
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84855-1-RR targets FOXP4 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | FOXP4 fusion protein Ag10047 相同性解析による交差性が予測される生物種 |
| Full Name | forkhead box P4 |
| Calculated molecular weight | 678 aa, 73 kDa |
| GenBank accession number | BC052803 |
| Gene Symbol | FOXP4 |
| Gene ID (NCBI) | 116113 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IVH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXP4 antibody 84855-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |