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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FPR2 Polyclonal antibody

FPR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30989-1-AP

Synonyms

FMLP-R-I, FMLP-related receptor I, FMLP related receptor I, FMLP R II, FMLP R I

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FPR2 Polyclonal antibody (30989-1-AP)

    WB analysis of HEK-293 using 30989-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 30989-1-AP (FPR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FPR2 Polyclonal antibody (30989-1-AP)

    IHC staining of mouse brain using 30989-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30989-1-AP (FPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30989-1-AP targets FPR2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FPR2 fusion protein Ag34304 相同性解析による交差性が予測される生物種
Full Name formyl peptide receptor 2
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight42 kDa
GenBank accession numberBC029125
Gene symbol FPR2
Gene ID (NCBI) 2358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The formyl peptide receptors (FPRs) are a group of G protein-coupled chemoattractant receptors that play important roles in host defense and inflammation (PMID: 28335409). In humans, three different isoforms are expressed (FPR1, FPR2, and FPR3). FPR2, also known as FPRL1 or ALX, is expressed by many cell types including monocytes, neutrophils, macrophages, astrocytoma cells, and epithelial cells. FPR2 conveys the biological functions of a variety of ligands, including N-formyl-methionyl peptides, the pro-resolution mediators annexin A1 (AnxA1) and lipoxin A4, as well as the activating and proinflammatory protein serum amyloid A (PMID: 22610094).

Protocols

Product Specific Protocols
WB protocol for FPR2 antibody 30989-1-APDownload protocol
IHC protocol for FPR2 antibody 30989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols