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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FREM1 Polyclonal antibody

FREM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13086-1-AP

Synonyms

FRAS1-related extracellular matrix protein 1, C9orf154, C9orf145, C9orf143

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using FREM1 Polyclonal antibody (13086-1-AP)

    WB analysis of human plasma using 13086-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using FREM1 Polyclonal antibody (13086-1-AP)

    WB analysis of HepG2 using 13086-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human liver cancer using 13086-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human liver cancer using 13086-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human breast cancer using 13086-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human breast cancer using 13086-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FREM1 Polyclonal antibody (13086-1-AP)

    IF Staining of HepG2 using 13086-1-AP

    Immunofluorescent analysis of HepG2 cells using 13086-1-AP (FREM1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma tissue, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13086-1-AP targets FREM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FREM1 fusion protein Ag3617 相同性解析による交差性が予測される生物種
Full Name FRAS1 related extracellular matrix 1
Calculated molecular weight2179aa, 244 kDa, 149.3 kDa
Observed molecular weight 40-43 kDa, 70-80 kDa
GenBank accession numberBC031064
Gene symbol FREM1
Gene ID (NCBI) 158326
RRIDAB_2877915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).

Protocols

Product Specific Protocols
WB protocol for FREM1 antibody 13086-1-APDownload protocol
IHC protocol for FREM1 antibody 13086-1-APDownload protocol
IF protocol for FREM1 antibody 13086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer.

Authors - Xiao-Yi Xu
View Article
humanIHC

Cancer Med

Elevated expression of FREM1 in breast cancer indicates favorable prognosis and high-level immune infiltration status.

Authors - Han-Ning Li
View Article