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  • Featured Product
  • KD/KO Validated

NCS1 Polyclonal antibody

NCS1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10506-2-AP

Synonyms

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of mouse brain using 10506-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of rat brain using 10506-2-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of mouse cerebellum using 10506-2-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of HepG2 using 10506-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of PC-3 using 10506-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of SH-SY5Y using 10506-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of human brain using 10506-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NCS1 Polyclonal antibody (10506-2-AP)

    WB analysis of A431 using 10506-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IP experiment of mouse brain using 10506-2-AP

    IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IHC staining of human cerebellum using 10506-2-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IHC staining of human cerebellum using 10506-2-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IHC staining of human brain using 10506-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IHC staining of human brain using 10506-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IF Staining of mouse cerebellum using 10506-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IF Staining of mouse cerebellum using 10506-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NCS1 Polyclonal antibody (10506-2-AP)

    IF Staining of mouse cerebellum using 10506-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using NCS1 Polyclonal antibody (10506-2-AP)

    FC experiment of SH-SY5Y using 10506-2-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10506-2-AP targets NCS1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCS1 fusion protein Ag0784 相同性解析による交差性が予測される生物種
Full Name frequenin homolog (Drosophila)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC004856
Gene symbol NCS1
Gene ID (NCBI) 23413
RRIDAB_2105918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

Protocols

Product Specific Protocols
WB protocol for NCS1 antibody 10506-2-APDownload protocol
IHC protocol for NCS1 antibody 10506-2-APDownload protocol
IF protocol for NCS1 antibody 10506-2-APDownload protocol
IP protocol for NCS1 antibody 10506-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory.

Authors - Bechara J Saab
View Article
mouseWB

Sci Rep

Self-directed exploration provides a Ncs1-dependent learning bonus.

Authors - Ho-Suk Mun
  • KD Validated
View Article
mouseWB,CoIP

Sci Transl Med

Activation of the sigma-1 receptor chaperone alleviates symptoms of Wolfram syndrome in preclinical models

Authors - Lucie Crouzier
View Article