Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16197-1-AP targets FRK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FRK fusion protein Ag10372 相同性解析による交差性が予測される生物種 |
| Full Name | fyn-related kinase |
| Calculated molecular weight | 505 aa, 56 kDa |
| Observed molecular weight | 54-57 kDa |
| GenBank accession number | BC012916 |
| Gene Symbol | FRK |
| Gene ID (NCBI) | 2444 |
| RRID | AB_10639525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named PTK5 (protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FRK antibody 16197-1-AP | Download protocol |
| IF protocol for FRK antibody 16197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia | ||
Discov Oncol Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |