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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FRS3 Polyclonal antibody

FRS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12249-1-AP

Synonyms

SNT 2, FRS2beta, FRS2B, FGFR-signaling adaptor SNT2, FGFR substrate 3

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using FRS3 Polyclonal antibody (12249-1-AP)

    WB analysis of human placenta using 12249-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12249-1-AP (FRS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using FRS3 Polyclonal antibody (12249-1-AP)

    IHC staining of human skin using 12249-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using FRS3 Polyclonal antibody (12249-1-AP)

    IHC staining of human skin using 12249-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FRS3 Polyclonal antibody (12249-1-AP)

    IF Staining of PC-3 using 12249-1-AP

    Immunofluorescent analysis of PC-3 cells using 12249-1-AP (FRS3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12249-1-AP targets FRS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRS3 fusion protein Ag2896 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor substrate 3
Calculated molecular weight 492 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010611
Gene symbol FRS3
Gene ID (NCBI) 10817
RRIDAB_2106244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FRS3 antibody 12249-1-APDownload protocol
IHC protocol for FRS3 antibody 12249-1-APDownload protocol
IF protocol for FRS3 antibody 12249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols