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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRZB Monoclonal antibody (67081-1-Ig)

    WB analysis using 67081-1-Ig (same clone as 67081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using FRZB Monoclonal antibody (67081-1-Ig)

    WB analysis of 4T1 using 67081-1-Ig (same clone as 67081-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67081-1-PBS in a different storage buffer formulation.

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FRZB Monoclonal antibody, PBS Only

FRZB Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67081-1-PBS
Clone No.2B4A1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67081-1-PBS targets FRZB in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FRZB fusion protein Ag28147 相同性解析による交差性が予測される生物種
Full Name frizzled-related protein
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027855
Gene Symbol FRZB
Gene ID (NCBI) 2487
RRIDAB_2882389
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92765
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.