Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 14384-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14384-1-AP
WB result of Fascin antibody (14384-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fascin transfected HeLa cells.
WB analysis using 14384-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14384-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 14384-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14384-1-AP (Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 14384-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14384-1-AP (Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of U-87 MG using 14384-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using Fascin antibody (14384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of SH-SY5Y using 14384-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Fascin (14384-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Featured Product
KD/KO Validated

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 14384-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

FSCN1, 55 kDa actin bundling protein, 55 kDa actin-bundling protein, FAN1, Fascin 1,Fascin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse thymus tissue, K-562 cells, NIH/3T3 cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected in	human tonsillitis tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	U-87 MG cells
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

14384-1-AP targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5816 Product name: Recombinant human Fascin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC000521 Sequence: MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYS 相同性解析による交差性が予測される生物種
Full Name	fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated molecular weight	55 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC000521
Gene Symbol	Fascin
Gene ID (NCBI)	6624
ENSEMBL Gene ID	ENSG00000075618
RRID	AB_2106381
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16658
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
FC protocol for Fascin antibody 14384-1-AP	Download protocol
IF protocol for Fascin antibody 14384-1-AP	Download protocol
IHC protocol for Fascin antibody 14384-1-AP	Download protocol
WB protocol for Fascin antibody 14384-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res The role of microRNA-133b and its target gene FSCN1 in gastric cancer. Authors - Lihua Guo View Article
mouse	WB,IF	Adv Sci (Weinh) Beyond Catalytic Therapy: Copper-Paeonol Nanozymes Disrupt Fascin-Mediated Actin Bundling to Suppress Tumor Growth and Metastasis. Authors - Peiying Zhang View Article
mouse,human	WB	Adv Sci (Weinh) The Histone Lysine Demethylase KDM7A Contributes to Reward Memory via Fscn1-Induced Synaptic Plasticity in the Medial Prefrontal Cortex Authors - Zhuo-Jin Yang View Article
human,mouse	WB	Adv Sci (Weinh) Cockroach-Derived Leucokinin VIII Peptide Accelerates Diabetic Skin Wound Healing by Enhancing Keratinocyte Filopodia Formation. Authors - Zhengshan Qin View Article
		Sci Adv NicheProt: Cell-type-resolved proteomics of tissue compartments. Authors - Yi-Chien Wu View Article
human	WB	Int J Biol Macromol Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro. Authors - Ajab Khan View Article

Validation Data Gallery

WB analysis using 14384-1-AP

WB analysis of HeLa using 14384-1-AP

WB analysis using 14384-1-AP

WB analysis of mouse thymus using 14384-1-AP

IHC staining of human tonsillitis using 14384-1-AP

IHC staining of human tonsillitis using 14384-1-AP

IHC staining of human liver cancer using 14384-1-AP

IHC staining of human liver cancer using 14384-1-AP

IF Staining of mouse brain using 14384-1-AP

IF Staining of mouse brain using 14384-1-AP

IF Staining of U-87 MG using 14384-1-AP

FC experiment of SH-SY5Y using 14384-1-AP

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The role of microRNA-133b and its target gene FSCN1 in gastric cancer.

Beyond Catalytic Therapy: Copper-Paeonol Nanozymes Disrupt Fascin-Mediated Actin Bundling to Suppress Tumor Growth and Metastasis.

The Histone Lysine Demethylase KDM7A Contributes to Reward Memory via Fscn1-Induced Synaptic Plasticity in the Medial Prefrontal Cortex

Cockroach-Derived Leucokinin VIII Peptide Accelerates Diabetic Skin Wound Healing by Enhancing Keratinocyte Filopodia Formation.

NicheProt: Cell-type-resolved proteomics of tissue compartments.

Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.

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