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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FSCN3 Polyclonal antibody

FSCN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13536-1-AP

Synonyms

Testis fascin, Fascin-3, Fascin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FSCN3 Polyclonal antibody (13536-1-AP)

    WB analysis of PC-3 using 13536-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13536-1-AP (FSCN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FSCN3 Polyclonal antibody (13536-1-AP)

    IHC staining of human prostate cancer using 13536-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FSCN3 Polyclonal antibody (13536-1-AP)

    IHC staining of human prostate cancer using 13536-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FSCN3 Polyclonal antibody (13536-1-AP)

    IF Staining of PC-3 using 13536-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13536-1-AP (FSCN3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FSCN3 Polyclonal antibody (13536-1-AP)

    IF Staining of PC-3 using 13536-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FSCN3 antibody (13536-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13536-1-AP targets FSCN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSCN3 fusion protein Ag4459 相同性解析による交差性が予測される生物種
Full Name fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus)
Calculated molecular weight 498 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC035606
Gene symbol FSCN3
Gene ID (NCBI) 29999
RRIDAB_2106517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FSCN3 antibody 13536-1-APDownload protocol
IHC protocol for FSCN3 antibody 13536-1-APDownload protocol
IF protocol for FSCN3 antibody 13536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols