Validation Data Gallery

Filter: AllWBIHC

WB analysis using 22665-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat ovary using 22665-1-AP
rat ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 22665-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 22665-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22665-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse ovary using 22665-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 22665-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

FSHR Polyclonal antibody

FSHR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22665-1-AP

Publications(60)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, ELISA

Synonyms

LGR1, FSHRO, FSH-R, FSH R, Follitropin receptor

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite®488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (60)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells
Positive IHC detected in	mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 51 publications below
IHC	See 16 publications below

Product Information

22665-1-AP targets FSHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FSHR fusion protein Ag18158 相同性解析による交差性が予測される生物種
Full Name	follicle stimulating hormone receptor
Calculated molecular weight	695 aa, 78 kDa
Observed molecular weight	71-78 kDa
GenBank accession number	BC118548
Gene Symbol	FSHR
Gene ID (NCBI)	2492
RRID	AB_2631204
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P23945
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for FSHR antibody 22665-1-AP	Download protocol
IHC protocol for FSHR antibody 22665-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	J Hazard Mater GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline Authors - Li Mou View Article
human,mouse	WB	Autophagy Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1 Authors - Tong Shao View Article
human,mouse	WB,IHC	Theranostics FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation Authors - Xingyan Xu View Article
mouse	WB	J Adv Res Core fucosylation regulates the ovarian response via FSH receptor during follicular development Authors - Tiantong Wang View Article
mouse	IHC	Redox Biol Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. Authors - Shotaro Hayashi View Article
mouse	WB	Cell Mol Life Sci P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1 Authors - Ting Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22665-1-AP

WB analysis of rat ovary using 22665-1-AP

WB analysis of DU 145 using 22665-1-AP

WB analysis of PC-3 using 22665-1-AP

WB analysis of HeLa using 22665-1-AP

IHC staining of mouse ovary using 22665-1-AP

IHC staining of mouse ovary using 22665-1-AP

FSHR Polyclonal antibody

FSHR Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline

Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation

Core fucosylation regulates the ovarian response via FSH receptor during follicular development

Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.

P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1