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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FSTL3 Polyclonal antibody (28218-1-AP)

    WB analysis using 28218-1-AP

    Human placenta tissues were subjected to SDS PAGE followed by western blot with 28218-1-AP (FSTL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FSTL3 Polyclonal antibody (28218-1-AP)

    WB analysis of HeLa using 28218-1-AP

    WB result of FSTL3 antibody (28218-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL3 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of human urothelial carcinoma using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of rat kidney using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of rat kidney using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of rat testis using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using FSTL3 Polyclonal antibody (28218-1-AP)

    IHC staining of rat testis using 28218-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

FSTL3/FLRG Polyclonal antibody

FSTL3/FLRG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28218-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, HeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28218-1-AP targets FSTL3/FLRG in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSTL3/FLRG fusion protein Ag27626 相同性解析による交差性が予測される生物種
Full Name follistatin-like 3 (secreted glycoprotein)
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight28-33 kDa
GenBank accession numberBC005839
Gene Symbol FSTL3
Gene ID (NCBI) 10272
RRIDAB_3086037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.

Protocols

Product Specific Protocols
WB protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
IHC protocol for FSTL3/FLRG antibody 28218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols