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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTCD Recombinant antibody (83664-2-RR)

    WB analysis using 83664-2-RR (same clone as 83664-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83664-2-RR (FTCD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83664-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00636-3

    Sandwich ELISA standard curve of MP00636-3

    Sandwich ELISA standard curve of MP00636-3, Human FTCD Recombinant Matched Antibody Pair - PBS only. 83664-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16646. 83664-4-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

  • Sample test of MP00636-3

    Sample test of MP00636-3

    The mean FTCD concentration was determined to be 114.0 pg/mL in HepG2 cell extract based on a 3.0 mg/mL extract load.

  • Flow cytometry (FC) experiment of HepG2 cells using FTCD Recombinant antibody (83664-2-RR)

    FC experiment of HepG2 using 83664-2-RR (same clone as 83664-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FTCD Recombinant antibody (83664-2-RR, Clone:240654G9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83664-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00636-1

    Cytometric bead array standard curve of MP00636-1

    Cytometric bead array standard curve of MP00636-1, FTCD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83664-2-PBS. Detection antibody: 83664-3-PBS. Standard: Ag16646. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83664-2-RR

    Affinity and Kinetic Characterization of 83664-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83664-2-RR against Human FTCD were performed. The affinity constant is 6.37 nM.

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FTCD Recombinant antibody, PBS Only (Capture)

FTCD Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83664-2-PBS
Clone No.240654G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83664-2-PBS targets FTCD as part of a matched antibody pair:

MP00636-1: 83664-2-PBS capture and 83664-3-PBS detection (validated in Cytometric bead array)

MP00636-3: 83664-2-PBS capture and 83664-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FTCD fusion protein Ag16646 相同性解析による交差性が予測される生物種
Full Name formiminotransferase cyclodeaminase
Calculated molecular weight 541 aa, 59 kDa
Observed molecular weight58 kDa
GenBank accession numberBC136395
Gene Symbol FTCD
Gene ID (NCBI) 10841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95954
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).