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Ferritin heavy chain Polyclonal antibody

Name: Ferritin heavy chain Polyclonal antibody
Brand: Proteintech
SKU: 11682-1-AP

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11682-1-AP

Synonyms

Ferritin, FTH1, FTH, Ferritin heavy chain, N-terminally processed, EC:1.16.3.1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 11682-1-AP
WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells.
WB analysis using 11682-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 11682-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 11682-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 11682-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11682-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11682-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 11682-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 11682-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HK-2 cells, HeLa cells, L02 cells, human placenta tissue
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11682-1-AP targets Ferritin heavy chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Ferritin heavy chain fusion protein Ag2290 相同性解析による交差性が予測される生物種
Full Name	ferritin, heavy polypeptide 1
Calculated molecular weight	183 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC000857
Gene symbol	FTH1
Gene ID (NCBI)	2495
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Protocols

Product Specific Protocols
WB protocol for Ferritin heavy chain antibody 11682-1-AP	Download protocol
IHC protocol for Ferritin heavy chain antibody 11682-1-AP	Download protocol
IF protocol for Ferritin heavy chain antibody 11682-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Inflammopharmacology Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis Authors - Chenyu Zhao View Article
mouse	WB	Mol Neurobiol A Disintegrin and Metalloproteinase-8 Protects Against Erastin-Induced Neuronal Ferroptosis via Activating Nrf2/HO-1/FTH1 Signaling Pathway Authors - Zhanyang Qian View Article
human	WB	BMC Cancer Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells Authors - Feifan Zhang View Article
human	WB	Aging (Albany NY) Eriocitrin inhibits epithelial-mesenchymal transformation (EMT) in lung adenocarcinoma cells via triggering ferroptosis Authors - Minglang Gao View Article
human	WB	Hum Exp Toxicol Protective effects of brain and muscle ARNT-like gene 1 on oxidized low-density lipoprotein-induced human brain microvascular endothelial cell injury by alleviating ferroptosis Authors - Shui Yu View Article
mouse	WB	Exp Cell Res A novel tsRNA-5008a promotes ferroptosis in cardiomyocytes that causes atrial structural remodeling predisposed to atrial fibrillation Authors - Liangzhen Xie View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ferritin heavy chain Polyclonal antibody

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 11682-1-AP

WB analysis using 11682-1-AP

WB analysis using 11682-1-AP

WB analysis using 11682-1-AP

IHC staining of mouse liver using 11682-1-AP

IHC staining of mouse liver using 11682-1-AP

IF Staining of HeLa using 11682-1-AP

IF Staining of HeLa using 11682-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis

A Disintegrin and Metalloproteinase-8 Protects Against Erastin-Induced Neuronal Ferroptosis via Activating Nrf2/HO-1/FTH1 Signaling Pathway

Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells

Eriocitrin inhibits epithelial-mesenchymal transformation (EMT) in lung adenocarcinoma cells via triggering ferroptosis

Protective effects of brain and muscle ARNT-like gene 1 on oxidized low-density lipoprotein-induced human brain microvascular endothelial cell injury by alleviating ferroptosis

A novel tsRNA-5008a promotes ferroptosis in cardiomyocytes that causes atrial structural remodeling predisposed to atrial fibrillation