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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FTSJ1 Polyclonal antibody

FTSJ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11620-1-AP

Synonyms

MRX44, JM23, FtsJ homolog 1 (E. coli), EC:2.1.1.205, CDLIV

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FTSJ1 Polyclonal antibody (11620-1-AP)

    WB analysis of HeLa using 11620-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11620-1-AP (FTSJ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using FTSJ1 Polyclonal antibody (11620-1-AP)

    IHC staining of human liver using 11620-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FTSJ1 Polyclonal antibody (11620-1-AP)

    IHC staining of human liver using 11620-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTSJ1 Polyclonal antibody (11620-1-AP)

    IHC staining of mouse brain using 11620-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTSJ1 Polyclonal antibody (11620-1-AP)

    IHC staining of mouse brain using 11620-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FTSJ1 Polyclonal antibody (11620-1-AP)

    IF Staining of A431 using 11620-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using FTSJ1 antibody (11620-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-87 MG cells
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11620-1-AP targets FTSJ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTSJ1 fusion protein Ag2199 相同性解析による交差性が予測される生物種
Full Name FtsJ homolog 1 (E. coli)
Calculated molecular weight 329 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC023584
Gene symbol FTSJ1
Gene ID (NCBI) 24140
RRIDAB_2877785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FTSJ1 antibody 11620-1-APDownload protocol
IHC protocol for FTSJ1 antibody 11620-1-APDownload protocol
IF protocol for FTSJ1 antibody 11620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Dengue virus exploits the host tRNA epitranscriptome to promote viral replication

Authors - Cheryl Chan
View Article