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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human skeletal muscle using 22556-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human placenta using 22556-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human placenta using 22556-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    IP experiment of mouse skeletal muscle using 22556-1-AP

    IP result of anti-FTSJ2 (IP:22556-1-AP, 3ug; Detection:22556-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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FTSJ2 Polyclonal antibody

FTSJ2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 22556-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

FJH1, FtsJ homolog 2 (E. coli), FTSJ2, Protein ftsJ homolog 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human placenta tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22556-1-AP targets FTSJ2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTSJ2 fusion protein Ag18410 相同性解析による交差性が予測される生物種
Full Name FtsJ homolog 2 (E. coli)
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC114514
Gene Symbol FTSJ2
Gene ID (NCBI) 29960
RRIDAB_2879122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9UI43
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Putative ribosomal RNA methyltransferase 2 (FTSJ2), also named as FJH1 or Protein FTSJ homolog 2 is a 246 amino acid protein, which belongs to the methyl-transferase superfamily. FTSJ2 localizes in the nucleus and is widely expressed in in muscle, placenta, and heart. FTSJ2 functions as a nucleolar RNA methyl-transferase involved in eukaryotic RNA processing and modification.

Protocols

Product Specific Protocols
WB protocol for FTSJ2 antibody 22556-1-APDownload protocol
IP protocol for FTSJ2 antibody 22556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols