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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTSJ3 Recombinant antibody (84311-6-RR)

    WB analysis using 84311-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84311-6-RR (FTSJ3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FTSJ3 Recombinant antibody (84311-6-RR)

    IF Staining of MCF-7 using 84311-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FTSJ3 antibody (84311-6-RR, Clone: 241507F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84311-6-RR

    Affinity and Kinetic Characterization of 84311-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84311-6-RR against Human FTSJ3 were performed. The affinity constant is 0.68 nM.

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FTSJ3 Recombinant antibody

FTSJ3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84311-6-RR
Clone No.241507F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SB92, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, FTSJ 3, EPCS3, EC:2.1.1.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  100 μL, 1000 μg/ml
20 μL, 1000 μg/ml
100 μL, 1000 μg/ml

¥85,000/ 100 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, SH-SY5Y cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84311-6-RR targets FTSJ3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FTSJ3 fusion protein Ag9835 相同性解析による交差性が予測される生物種
Full Name FtsJ homolog 3 (E. coli)
Calculated molecular weight 847 aa, 97 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC036710
Gene Symbol FTSJ3
Gene ID (NCBI) 117246
RRIDAB_3671851
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Protocols

Product Specific Protocols
WB protocol for FTSJ3 antibody 84311-6-RRDownload protocol
IF protocol for FTSJ3 antibody 84311-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols