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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUCA1 Monoclonal antibody, PBS Only

FUCA1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat no : 67251-1-PBS

Synonyms

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of K-562 using 67251-1-Ig (same clone as 67251-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of NIH/3T3 using 67251-1-Ig (same clone as 67251-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of HEK-293 using 67251-1-Ig (same clone as 67251-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67251-1-PBS targets FUCA1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FUCA1 fusion protein Ag9638 相同性解析による交差性が予測される生物種
Full Name fucosidase, alpha-L- 1, tissue
Calculated molecular weight 461 aa, 53 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC017338
Gene symbol FUCA1
Gene ID (NCBI) 2517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).