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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUCA2 Polyclonal antibody

FUCA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30080-1-AP

Synonyms

Alpha L fucosidase 2, dJ20N2.5, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUCA2 Polyclonal antibody (30080-1-AP)

    WB analysis using 30080-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FUCA2 Polyclonal antibody (30080-1-AP)

    IHC staining of human stomach cancer using 30080-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FUCA2 Polyclonal antibody (30080-1-AP)

    IHC staining of human stomach cancer using 30080-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FUCA2 Polyclonal antibody (30080-1-AP)

    IF Staining of HepG2 using 30080-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30080-1-AP targets FUCA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUCA2 fusion protein Ag32467 相同性解析による交差性が予測される生物種
Full Name fucosidase, alpha-L- 2, plasma
Calculated molecular weight 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC003060
Gene symbol FUCA2
Gene ID (NCBI) 2519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

Protocols

Product Specific Protocols
WB protocol for FUCA2 antibody 30080-1-APDownload protocol
IHC protocol for FUCA2 antibody 30080-1-APDownload protocol
IF protocol for FUCA2 antibody 30080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols