Validation Data Gallery
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Tested Applications
| Positive WB detected in | RAW 264.7 cells, RAW 264, .7 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
28519-1-AP targets FUNDC1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, monkey, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FUNDC1 fusion protein Ag29358 相同性解析による交差性が予測される生物種 |
| Full Name | FUN14 domain containing 1 |
| Calculated molecular weight | 155 aa, 17 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC042813 |
| Gene Symbol | FUNDC1 |
| Gene ID (NCBI) | 139341 |
| RRID | AB_2935469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FUNDC1 is a novel mitochondrial-associated membrane (MAM) protein, enriched at the MAM by interacting with the ER resident protein CANX (calnexin) under hypoxia. As mitophagy proceeds, it dissociates from CANX and preferably recruits DNM1L/DRP1 to drive mitochondrial fission in response to hypoxic stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUNDC1 antibody 28519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Sci (Lond) Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning
| ||
FASEB J Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system | ||
Mech Ageing Dev Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice | ||
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1 | ||
J Exp Clin Cancer Res Synthetic lethality of combined ULK1 defection and p53 restoration induce pyroptosis by directly upregulating GSDME transcription and cleavage activation through ROS/NLRP3 signaling | ||
PLoS Pathog Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation |