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  • Featured Product
  • KD/KO Validated

FUS/TLS Monoclonal antibody, PBS Only

FUS/TLS Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B4F8

Cat no : 68262-1-PBS

Synonyms

FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

    Untreated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis of Jurkat using 68262-1-Ig (same clone as 68262-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis of Neuro-2a using 68262-1-Ig (same clone as 68262-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis of HepG2 using 68262-1-Ig (same clone as 68262-1-PBS)

    WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUS/TLS transfected HepG2 cells. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FUS/TLS Monoclonal antibody (68262-1-Ig)

    WB analysis of HeLa using 68262-1-Ig (same clone as 68262-1-PBS)

    WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; room temperature for 1.5 hours) with negative control and FUS/TLS knockout HeLa cells. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of human colon using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of human ovary tumor using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FUS/TLS Monoclonal antibody (68262-1-Ig)

    IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using FUS/TLS Monoclonal antibody (68262-1-Ig)

    FC experiment of HEK-293T using 68262-1-Ig (same clone as 68262-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human FUS/TLS (68262-1-Ig, Clone:1B4F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68262-1-PBS targets FUS/TLS in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FUS/TLS fusion protein Ag2150 相同性解析による交差性が予測される生物種
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight 75 kDa
Observed molecular weight 53 kDa, 68-75 kDa
GenBank accession numberBC026062
Gene symbol FUS/TLS
Gene ID (NCBI) 2521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).