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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUT2 Polyclonal antibody (20288-1-AP)

    WB analysis using 20288-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20288-1-AP (FUT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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FUT2 Polyclonal antibody

FUT2 Polyclonal Antibody for WB, ELISA
Cat No. 20288-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Alpha(1,2)FT 2, B12QTL1, Fucosyltransferase 2, FUT2, SE, Se2, SEC2, Secretor factor, sej

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Calu-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20288-1-AP targets FUT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT2 fusion protein Ag14047 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 2 (secretor status included)
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight35-39 kDa
GenBank accession numberBC001899
Gene Symbol FUT2
Gene ID (NCBI) 2524
RRIDAB_3669331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10981
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fucosyltransferase 2 (FUT2) is a key enzyme that catalyzes the transfer of fucose to the terminal galactose of type 1 or type 2 disaccharide via α1,2-linkage. FUT2 gene codes for the alpha(1,2)fucosyltransferase responsible for the synthesis of the H antigen, which is the precursor of the ABO histo-blood group antigens in body fluids and on the intestinal mucosa (PMID: 19487333). FUT2 is highly expressed in lung adenocarcinoma (LUAD) and plays a vital role in the tumorigenesis of LUAD (PMID: 36552800). FUT2 has a calculated molecular mass of ~39 kDa, and the 70kDa band might be the glycosylated form.

Protocols

Product Specific Protocols
WB protocol for FUT2 antibody 20288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols