Featured Product
KD/KO Validated

FUT4 Polyclonal antibody

Name: FUT4 Polyclonal antibody
Brand: Proteintech
SKU: 19497-1-AP

FUT4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19497-1-AP

Synonyms

FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, Alpha-(1,3)-fucosyltransferase 4

Validation Data Gallery

Filter:

AllWBIPIHCFC (Intra)

WB analysis using 19497-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19497-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 19497-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19497-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19497-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19497-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19497-1-AP
IP result of anti-FUT4 (IP:19497-1-AP, 4ug; Detection:19497-1-AP 1:500) with HeLa cells lysate 2480ug.
IHC staining of human lung cancer using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19497-1-AP (FUT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin cancer using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human gliomas using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 19497-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:50 (under 40x lens).
FC experiment of A375 using 19497-1-AP
1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human FUT4 (19497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

-/ -

Cat No. 19497-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, HL-60 cells, HepG2 cells, SGC-7901 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human gliomas tissue, human skin cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 18 publications below
IHC	See 7 publications below
IF	See 10 publications below

Product Information

19497-1-AP targets FUT4 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Calculated molecular weight	59 kDa
Observed molecular weight	95-140 kDa
GenBank accession number	NM_002033
Gene symbol	FUT4
Gene ID (NCBI)	2526
RRID	AB_10733222
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 19497-1-AP antibody detects the glycosylated isoform proteins around 95-140 kDa in SDS-PAGE. (PMID: 28706275, 28914881, 11278338)

Protocols

Product Specific Protocols
WB protocol for FUT4 antibody 19497-1-AP	Download protocol
IHC protocol for FUT4 antibody 19497-1-AP	Download protocol
IP protocol for FUT4 antibody 19497-1-AP	Download protocol
FC protocol for FUT4 antibody 19497-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	ACS Appl Mater Interfaces Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair. Authors - Lei Fan View Article
human	WB	Cell Death Dis α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation Authors - Hao Wang View Article
human,mouse	WB,IF	Cell Death Differ MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation. Authors - Qin Zheng KD Validated View Article
human	WB,IF	Oncotarget Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4. Authors - Lili Tian KD Validated View Article
human	WB	Cell Death Dis Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells. Authors - Yang X X KD Validated View Article
human	IF	Front Mol Neurosci Transcriptional Repression of p53 by PAX3 Contributes to Gliomagenesis and Differentiation of Glioma Stem Cells. Authors - Hui Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUT4 Polyclonal antibody

FUT4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 19497-1-AP

WB analysis of HeLa using 19497-1-AP

WB analysis of HL-60 using 19497-1-AP

WB analysis of Jurkat using 19497-1-AP

WB analysis of HepG2 using 19497-1-AP

WB analysis of Jurkat using 19497-1-AP

IP experiment of HeLa using 19497-1-AP

IHC staining of human lung cancer using 19497-1-AP

IHC staining of human lung cancer using 19497-1-AP

IHC staining of human stomach cancer using 19497-1-AP

IHC staining of human skin cancer using 19497-1-AP

IHC staining of human skin cancer using 19497-1-AP

IHC staining of human gliomas using 19497-1-AP

IHC staining of human gliomas using 19497-1-AP

FC experiment of A375 using 19497-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair.

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation.

Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4.

Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells.

Transcriptional Repression of p53 by PAX3 Contributes to Gliomagenesis and Differentiation of Glioma Stem Cells.