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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUT4 Polyclonal antibody

FUT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22141-1-AP

Synonyms

FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, Alpha-(1,3)-fucosyltransferase 4

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FUT4 Polyclonal antibody (22141-1-AP)

    WB analysis of Jurkat using 22141-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using FUT4 Polyclonal antibody (22141-1-AP)

    WB analysis of A431 using 22141-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FUT4 Polyclonal antibody (22141-1-AP)

    IHC staining of human lung cancer using 22141-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FUT4 Polyclonal antibody (22141-1-AP)

    IHC staining of human lung cancer using 22141-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FUT4 Polyclonal antibody (22141-1-AP)

    IF Staining of A431 using 22141-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FUT4 antibody (22141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, A431 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22141-1-AP targets FUT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT4 fusion protein Ag17565 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Calculated molecular weight 530 aa, 59 kDa
Observed molecular weight 45 kDa, 63 kDa
GenBank accession numberBC136374
Gene symbol FUT4
Gene ID (NCBI) 2526
RRIDAB_2879006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 22141-1-AP detects the band around 45 kDa and glycosylated isoform 63 kDa protein in SDS-PAGE. (PMID: 22287018, 17335083, 11278338)

Protocols

Product Specific Protocols
WB protocol for FUT4 antibody 22141-1-APDownload protocol
IHC protocol for FUT4 antibody 22141-1-APDownload protocol
IF protocol for FUT4 antibody 22141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols