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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

FUT6 Polyclonal antibody

FUT6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16567-1-AP

Synonyms

Alpha (1,3) fucosyltransferase, FCT3A, FT1A, Fuc TVI, Fucosyltransferase 6, Fucosyltransferase VI, FucT VI, FUT6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FUT6 Polyclonal antibody (16567-1-AP)

    WB analysis of HeLa using 16567-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16567-1-AP (FUT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FUT6 Polyclonal antibody (16567-1-AP)

    WB analysis of HeLa using 16567-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16567-1-AP (FUT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FUT6 Polyclonal antibody (16567-1-AP)

    IF Staining of HeLa using 16567-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16567-1-AP (FUT6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16567-1-AP targets FUT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT6 fusion protein Ag9847 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 6 (alpha (1,3) fucosyltransferase)
Calculated molecular weight 359 aa, 42 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC061700
Gene symbol FUT6
Gene ID (NCBI) 2528
RRIDAB_2105683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FUT6 antibody 16567-1-APDownload protocol
IF protocol for FUT6 antibody 16567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

IUBMB Life

MicroRNA-106b targets FUT6 to promote cell migration, invasion, and proliferation in human breast cancer.

Authors - Nana Li
  • KD Validated
View Article
humanWB

Biochim Biophys Acta Mol Cell Res

HOTAIR/miR-326/FUT6 axis facilitates colorectal cancer progression through regulating fucosylation of CD44 via PI3K/AKT/mTOR pathway.

Authors - Shimeng Pan
View Article
humanWB,IHC

Transl Oncol

A functional variant rs10409772 in FUT6 promoter regulates colorectal cancer progression through PKA/CREB signaling

Authors - Jie Zhang
View Article