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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUT8 Polyclonal antibody

FUT8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29430-1-AP

Synonyms

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUT8 Polyclonal antibody (29430-1-AP)

    WB analysis using 29430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29430-1-AP (FUT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FUT8 Polyclonal antibody (29430-1-AP)

    IHC staining of human lung cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT8 Polyclonal antibody (29430-1-AP)

    IHC staining of human breast cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMDA-MB-231 cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29430-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT8 fusion protein Ag30784 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Calculated molecular weight 66 kDa
Observed molecular weight60-66 kDa
GenBank accession numberBC025385
Gene symbol FUT8
Gene ID (NCBI) 2530
RRIDAB_2918305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FUT8 antibody 29430-1-APDownload protocol
IHC protocol for FUT8 antibody 29430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols