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Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of pig brain using 60230-1-Ig (same clone as 60230-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of fetal human brain using 60230-1-Ig (same clone as 60230-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of rat brain using 60230-1-Ig (same clone as 60230-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of mouse brain using 60230-1-Ig (same clone as 60230-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IF Staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FUT9 antibody (60230-1-Ig, Clone: 5D4G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FUT9 Monoclonal antibody, PBS Only

FUT9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60230-1-PBS
Clone No.5D4G6

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60230-1-PBS targets FUT9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FUT9 fusion protein Ag8298 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
Calculated molecular weight 359 aa, 42 kDa
Observed molecular weight 44-46 kDa
GenBank accession numberBC036101
Gene Symbol FUT9
Gene ID (NCBI) 10690
RRIDAB_11183046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y231
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.