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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50628-1

    Sandwich ELISA standard curve of MP50628-1

    Sandwich ELISA standard curve of MP50628-1, Human FXN Monoclonal Matched Antibody Pair - PBS only. 68925-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6632. 68925-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP50628-1

    Sample test of MP50628-1

    The mean FXN concentration was determined to be 246.06 ng/mL in HL-60 cell extract based on a 1.4 mg/mL extract load, 46.90 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load and 476.28 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP50628-1

    Cytometric bead array standard curve of MP50628-1

    Cytometric bead array standard curve of MP50628-1, FXN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68925-2-PBS. Detection antibody: 68925-3-PBS. Standard:Ag6632. Range: 0.098-100 ng/mL.

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FXN Monoclonal antibody, PBS Only (Capture)

FXN Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68925-2-PBS
Clone No.2D4C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Extramitochondrial frataxin, EC:1.16.3.1, dFXN, d FXN, CyaY

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68925-2-PBS targets FXN as part of a matched antibody pair:

MP50628-1: 68925-2-PBS capture and 68925-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FXN fusion protein Ag6632 相同性解析による交差性が予測される生物種
Full Name frataxin
Calculated molecular weight 23 kDa
GenBank accession numberBC048097
Gene Symbol FXN
Gene ID (NCBI) 2395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G Magarose purification
UNIPROT IDQ16595
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.