Featured Product
KD/KO Validated

FXR2 Polyclonal antibody

Name: FXR2 Polyclonal antibody
Brand: Proteintech
SKU: 12552-1-AP

FXR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12552-1-AP

Synonyms

RNA-binding protein FXR2, FXR2P, FMR1L2, FMR1 autosomal homolog 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 12552-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 12552-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12552-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12552-1-AP
IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.
IHC staining of mouse brain using 12552-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12552-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12552-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 12552-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

-/ -

Cat No. 12552-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, human brain tissue, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
RIP	See 1 publications below

Product Information

12552-1-AP targets FXR2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FXR2 fusion protein Ag3402 相同性解析による交差性が予測される生物種
Full Name	fragile X mental retardation, autosomal homolog 2
Calculated molecular weight	74 kDa
Observed molecular weight	95 kDa
GenBank accession number	BC020090
Gene symbol	FXR2
Gene ID (NCBI)	9513
RRID	AB_2110720
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FXR2 (FMR1 autosomal homolog 2), also known as: FXR2P; Fxr2h, is belong to the Fragile X gene family. FXR2 is a mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis. Also, FXR2 acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus(Uniprot).

Protocols

Product Specific Protocols
WB protocol for FXR2 antibody 12552-1-AP	Download protocol
IHC protocol for FXR2 antibody 12552-1-AP	Download protocol
IF protocol for FXR2 antibody 12552-1-AP	Download protocol
IP protocol for FXR2 antibody 12552-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Biol UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope Authors - Yongrong Liao View Article
human	RIP	Elife The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. Authors - Belinda Baquero-Perez View Article
human	WB	Proc Natl Acad Sci U S A ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation Authors - Gui-Feng Zhou KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FXR2 Polyclonal antibody

FXR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 12552-1-AP

WB analysis using 12552-1-AP

WB analysis of human brain using 12552-1-AP

IP experiment of HeLa using 12552-1-AP

IHC staining of mouse brain using 12552-1-AP

IHC staining of mouse brain using 12552-1-AP

IF Staining of HepG2 using 12552-1-AP