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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FXYD2 Recombinant antibody

FXYD2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240649C2

Cat no : 83860-1-RR

Synonyms

Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using FXYD2 Recombinant antibody (83860-1-RR)

    WB analysis of human kidney using 83860-1-RR

    human kidney tissue were subjected to SDS PAGE followed by western blot with 83860-1-RR (FXYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using FXYD2 Recombinant antibody (83860-1-RR)

    FC experiment of HepG2 using 83860-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FXYD2 Recombinant antibody (83860-1-RR, Clone:240649C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83860-1-RR

    Affinity and Kinetic Characterization of 83860-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83860-1-RR against Human FXYD2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inhuman kidney tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83860-1-RR is FXYD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FXYD2 fusion protein Ag34030 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 2
Calculated molecular weight 7 kDa
Observed molecular weight 7-10 kDa
GenBank accession numberBC013289
Gene symbol FXYD2
Gene ID (NCBI) 486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Protocols

Product Specific Protocols
WB protocol for FXYD2 antibody 83860-1-RRDownload protocol
FC protocol for FXYD2 antibody 83860-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols