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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FXYD6 Monoclonal antibody, PBS Only

FXYD6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1B7

Cat no : 68058-1-PBS

Synonyms

FXYD6, Phosphohippolin

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FXYD6 Monoclonal antibody (68058-1-Ig)

    WB analysis using 68058-1-Ig (same clone as 68058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68058-1-Ig (FXYD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FXYD6 Monoclonal antibody (68058-1-Ig)

    WB analysis using 68058-1-Ig (same clone as 68058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68058-1-Ig (FXYD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse brain using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXYD6 Monoclonal antibody (68058-1-Ig)

    IHC staining of mouse brain using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using FXYD6 Monoclonal antibody (68058-1-Ig)

    IF Staining of PC-12 using 68058-1-Ig (same clone as 68058-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using FXYD6 antibody (68058-1-Ig, Clone: 2H1B7 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68058-1-PBS targets FXYD6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FXYD6 fusion protein Ag8538 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 6
Calculated molecular weight 95 aa, 11 kDa
Observed molecular weight20 kDa
GenBank accession numberBC018652
Gene symbol FXYD6
Gene ID (NCBI) 53826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.