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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FXYD7 Polyclonal antibody (11465-1-AP)

    WB analysis of mouse brain using 11465-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11465-1-AP (FXYD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FXYD7 Polyclonal antibody (11465-1-AP)

    WB analysis of human brain using 11465-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11465-1-AP (FXYD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using FXYD7 Polyclonal antibody (11465-1-AP)

    WB analysis of rat brain using 11465-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11465-1-AP (FXYD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FXYD7 Polyclonal antibody (11465-1-AP)

    WB analysis of human brain using 11465-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11465-1-AP (FXYD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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FXYD7 Polyclonal antibody

FXYD7 Polyclonal Antibody for WB, ELISA
Cat No. 11465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

FXYD7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11465-1-AP targets FXYD7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXYD7 fusion protein Ag2030 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 7
Calculated molecular weight 9 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC018619
Gene Symbol FXYD7
Gene ID (NCBI) 53822
RRIDAB_2108627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD7 (FXYD domain containing ion transport regulator 7) is a single-pass membrane protein that belongs to the FXYD family. The FXYD family, which contains seven members, are tissue specific regulators of the Na,K-ATPase. Expressed exclusively in the brain, FXYD7 is an isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability. It has been reported that FXYD7 bears post-translationally added modifications on threonine residues and has an apparent molecular weight of 18 kDa (PMID: 12093728).

Protocols

Product Specific Protocols
WB protocol for FXYD7 antibody 11465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols