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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FYB Polyclonal antibody (31553-1-AP)

    WB analysis using 31553-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 31553-1-AP (FYB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FYB Polyclonal antibody (31553-1-AP)

    IHC staining of human ovary cancer using 31553-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31553-1-AP (FYB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FYB Polyclonal antibody

FYB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31553-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SLAP130, SLAP 130, p120/p130, FYB 120/130, ADAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31553-1-AP targets FYB in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FYB fusion protein Ag35929 相同性解析による交差性が予測される生物種
Full Name FYN binding protein (FYB-120/130)
Calculated molecular weight85kDa，783aa
Observed molecular weight110 kDa
GenBank accession numberNM_199335.3
Gene Symbol FYB
Gene ID (NCBI) 2533
RRIDAB_3670034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15117
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FYN binding protein (FYB-120/130), also known as FYB, ADAP (Adhesion and degranulation-promoting adapter protein), and SLAP-130 (SLP-76-associated phosphoprotein) is a protein that is encoded by the FYB gene in humans. The protein is highly expressed in T cells, NK cells, bone marrow cells, and platelets, responsible for the signal transduction upon T cell receptor (TCR) stimulation with integrin activation.

Protocols

Product Specific Protocols
WB protocol for FYB antibody 31553-1-APDownload protocol
IHC protocol for FYB antibody 31553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols