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Species Reactivity

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  • Featured Product
  • KD/KO Validated

FYCO1 Polyclonal antibody

FYCO1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25730-1-AP

Synonyms

FYCO1, RUFY3, ZFYVE7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FYCO1 Polyclonal antibody (25730-1-AP)

    WB analysis using 25730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25730-1-AP (FYCO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FYCO1 Polyclonal antibody (25730-1-AP)

    WB analysis of HeLa using 25730-1-AP

    WB result of FYCO1 antibody (25730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYCO1 transfected HeLa cells.

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FYCO1 fusion protein Ag22803 相同性解析による交差性が予測される生物種
Full Name FYVE and coiled-coil domain containing 1
Calculated molecular weight 1478 aa, 167 kDa
Observed molecular weight167-180 kDa
GenBank accession numberBC007218
Gene symbol FYCO1
Gene ID (NCBI) 79443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

Protocols

Product Specific Protocols
WB protocol for FYCO1 antibody 25730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF, WB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
View Article