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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FYTTD1 Polyclonal antibody (24560-1-AP)

    WB analysis of HeLa using 24560-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24560-1-AP (FYTTD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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FYTTD1 Polyclonal antibody

FYTTD1 Polyclonal Antibody for WB, ELISA
Cat No. 24560-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FYTTD1, Protein 40 2 3, UAP56 interacting factor, UIF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24560-1-AP targets FYTTD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FYTTD1 fusion protein Ag20051 相同性解析による交差性が予測される生物種
Full Name forty-two-three domain containing 1
Calculated molecular weight 318 aa, 36 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC039734
Gene Symbol FYTTD1
Gene ID (NCBI) 84248
RRIDAB_2879609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FYTTD1 also termed as UAP56 interacting factor or UIF is a 318 amino acid protein, which belongs to the UIF family. FYTTD1 localizes to nucleus and is required for mRNA export from nucleus to cytoplasm. Functioning as an adaptor, FYTTD1 utilizes the BAT1/DDX39-TAP pathway, which is essential for efficient mRNA export and nuclear pore delivery. FYTTD1 interacts with SSRP1, a protein that is necessary for its recruitment of mRNAs, in addition to a mutually exclusive interaction with BAT1/DDX39 and TAP. The molecular weight of post-translation modified FYTTD1 is observed 50 kDa.

Protocols

Product Specific Protocols
WB protocol for FYTTD1 antibody 24560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols