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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FZD1 Polyclonal antibody (28326-1-AP)

    WB analysis of HeLa using 28326-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28326-1-AP (FZD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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FZD1 Polyclonal antibody

FZD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28326-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

DKFZp564G072, FLJ95923, Frizzled 1, Fz 1, FZD1, FzE1, hFz1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28326-1-AP targets FZD1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FZD1 fusion protein Ag28500 相同性解析による交差性が予測される生物種
Full Name frizzled homolog 1 (Drosophila)
Calculated molecular weight 647 aa, 71 kDa
GenBank accession numberBC051271
Gene Symbol FZD1
Gene ID (NCBI) 8321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UP38
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.