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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Frizzled 9 Polyclonal antibody

Frizzled 9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13865-1-AP

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis using 13865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse testis using 13865-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse testis using 13865-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse skeletal muscle using 13865-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of HeLa using 13865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse brain using 13865-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of mouse eye using 13865-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Frizzled 9 Polyclonal antibody (13865-1-AP)

    WB analysis of HUVEC using 13865-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    IHC staining of human gliomas using 13865-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Frizzled 9 Polyclonal antibody (13865-1-AP)

    IHC staining of human gliomas using 13865-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, mouse testis tissue, mouse brain tissue, mouse eye tissue, HUVEC cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13865-1-AP targets Frizzled 9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Frizzled 9 fusion protein Ag4847 相同性解析による交差性が予測される生物種
Full Name frizzled homolog 9 (Drosophila)
Calculated molecular weight 591 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC026333
Gene symbol Frizzled 9
Gene ID (NCBI) 8326
RRIDAB_2107358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Frizzled 9 antibody 13865-1-APDownload protocol
IHC protocol for Frizzled 9 antibody 13865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.

Authors - Yang Bai
View Article
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu
View Article