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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Factor IX/PTC Polyclonal antibody (32051-1-AP)

    WB analysis using 32051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32051-1-AP (Factor IX/PTC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Factor IX/PTC Polyclonal antibody (32051-1-AP)

    IHC staining of mouse liver using 32051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32051-1-AP (Factor IX/PTC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Factor IX/PTC Polyclonal antibody

Factor IX/PTC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32051-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, L02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32051-1-AP targets Factor IX/PTC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Factor IX/PTC fusion protein Eg1045 相同性解析による交差性が予測される生物種
Full Name coagulation factor IX
Calculated molecular weight52kDa
Observed molecular weight30-33 kDa, 50 kDa
GenBank accession numberNM_000133.4
Gene Symbol F9
Gene ID (NCBI) 2158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP00740-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond.

Protocols

Product Specific Protocols
WB protocol for Factor IX/PTC antibody 32051-1-APDownload protocol
IHC protocol for Factor IX/PTC antibody 32051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols