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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Factor XII Monoclonal antibody, PBS Only

Factor XII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E2F11

Cat no : 66089-1-PBS

Synonyms

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using Factor XII Monoclonal antibody (66089-1-Ig)

    WB analysis of K-562 using 66089-1-Ig (same clone as 66089-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor XII Monoclonal antibody (66089-1-Ig)

    IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor XII Monoclonal antibody (66089-1-Ig)

    IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Factor XII Monoclonal antibody (66089-1-Ig)

    IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Factor XII Monoclonal antibody (66089-1-Ig)

    IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Factor XII Monoclonal antibody (66089-1-Ig)

    IF Staining of mouse liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Factor XII Monoclonal antibody (66089-1-Ig)

    IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Factor XII Monoclonal antibody (66089-1-Ig)

    IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66089-1-PBS targets Factor XII in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XII fusion protein Ag3401 相同性解析による交差性が予測される生物種
Full Name coagulation factor XII (Hageman factor)
Calculated molecular weight 615 aa, 68 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC012390
Gene symbol Factor XII
Gene ID (NCBI) 2161
RRIDAB_11232031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.