• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

Fas/CD95 Polyclonal antibody, PBS Only

Fas/CD95 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13098-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

    WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Fas/CD95 Polyclonal antibody (13098-1-AP)

    IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

    IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13098-1-PBS targets Fas/CD95 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fas/CD95 fusion protein Ag3718 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.