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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

    WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (13098-1-AP)

    WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Fas/CD95 Polyclonal antibody (13098-1-AP)

    IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

    IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13098-1-PBS targets Fas/CD95 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fas/CD95 fusion protein Ag3718 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene Symbol Fas/CD95
Gene ID (NCBI) 355
RRIDAB_2278042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25445
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.