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Tested Applications
| Positive WB detected in | Jurkat cells, J774A.1 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30601-1-AP targets Fas/CD95 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fas/CD95 fusion protein Ag30426 相同性解析による交差性が予測される生物種 |
| Full Name | Fas (TNF receptor superfamily member 6) |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 30-40 kDa |
| GenBank accession number | NM_007987 |
| Gene Symbol | Fas/CD95 |
| Gene ID (NCBI) | 14102 |
| RRID | AB_3086371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25446 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Fas/CD95 antibody 30601-1-AP | Download protocol |
| IF protocol for Fas/CD95 antibody 30601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |