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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fas/CD95 Polyclonal antibody

Fas/CD95 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30601-1-AP

Synonyms

Fas, CD95, TNFR6, APT1, APO 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (30601-1-AP)

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (30601-1-AP)

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using Fas/CD95 Polyclonal antibody (30601-1-AP)

    IF Staining of Raji using 30601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, J774A.1 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30601-1-AP targets Fas/CD95 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fas/CD95 fusion protein Ag30426 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily member 6)
Calculated molecular weight 37 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberNM_007987
Gene symbol Fas/CD95
Gene ID (NCBI) 14102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

Protocols

Product Specific Protocols
WB protocol for Fas/CD95 antibody 30601-1-APDownload protocol
IF protocol for Fas/CD95 antibody 30601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols