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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Fas/CD95 Rabbit Recombinant Antibody

Fas/CD95 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240340G11

Cat no : 98034-2-RR

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR)

    FC experiment of human PBMCs using 98034-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR)

    FC experiment of human PBMCs using 98034-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98034-2-RR

    Affinity and Kinetic Characterization of 98034-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98034-2-RR against Human Fas/CD95 were performed. The affinity constant is 0.191 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98034-2-RR is Fas/CD95 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight38 kDa
GenBank accession numberNM_000043.6
Gene symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

Protocols

Product Specific Protocols
FC protocol for Fas/CD95 antibody 98034-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols