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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fas/CD95 Recombinant antibody, PBS Only

Fas/CD95 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240340E11

Cat no : 83400-5-PBS

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Raji cells using Fas/CD95 Recombinant antibody (83400-5-RR)

    IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using Fas/CD95 Recombinant antibody (83400-5-RR)

    IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Fas/CD95 Recombinant antibody (83400-5-RR)

    IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Fas/CD95 Recombinant antibody (83400-5-RR)

    IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83400-5-RR

    Affinity and Kinetic Characterization of 83400-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83400-5-PBS is Fas/CD95 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight38 kDa
GenBank accession numberNM_000043.6
Gene symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.