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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ferritin light chain Monoclonal antibody (68068-1-Ig)

    IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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Ferritin light chain Monoclonal antibody, PBS Only

Ferritin light chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68068-1-PBS
Clone No.1F9F5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68068-1-PBS targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ferritin light chain fusion protein Ag20637 相同性解析による交差性が予測される生物種
Full Name ferritin, light polypeptide
Calculated molecular weight 19 kDa
Observed molecular weight18 kDa
GenBank accession numberBC008439
Gene Symbol Ferritin light chain
Gene ID (NCBI) 2512
RRIDAB_2918809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02792
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.