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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fibrillin 1 Polyclonal antibody

Fibrillin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29425-1-AP

Synonyms

FBN1, MFS1, MASS, Fibrillin-1, FBN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fibrillin 1 Polyclonal antibody (29425-1-AP)

    WB analysis using 29425-1-AP

    HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Fibrillin 1 Polyclonal antibody (29425-1-AP)

    IHC staining of human endometrial cancer using 29425-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Fibrillin 1 Polyclonal antibody (29425-1-AP)

    IHC staining of human endometrial cancer using 29425-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Fibrillin 1 Polyclonal antibody (29425-1-AP)

    IHC staining of human normal colon using 29425-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Fibrillin 1 Polyclonal antibody (29425-1-AP)

    IF Staining of A431 using 29425-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29425-1-AP targets Fibrillin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibrillin 1 fusion protein Ag30283 相同性解析による交差性が予測される生物種
Full Name fibrillin 1
Observed molecular weight350 kDa
GenBank accession numberBC146854
Gene symbol FBN1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).

Protocols

Product Specific Protocols
WB protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IHC protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IF protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols