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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fibrillin 1 Recombinant antibody

Fibrillin 1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240960G12

Cat no : 83836-4-RR

Synonyms

FBN1, MASS, Fibrillin-1, FBN, Asprosin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Fibrillin 1 Recombinant antibody (83836-4-RR)

    WB analysis of HeLa using 83836-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Fibrillin 1 Recombinant antibody (83836-4-RR)

    IHC staining of human stomach cancer using 83836-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83836-4-RR

    Affinity and Kinetic Characterization of 83836-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83836-4-RR against Human Fibrillin 1 were performed. The affinity constant is 51.3 nM.

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83836-4-RR is Fibrillin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fibrillin 1 fusion protein Ag30283 相同性解析による交差性が予測される生物種
Full Name fibrillin 1
Observed molecular weight350 kDa
GenBank accession numberBC146854
Gene symbol Fibrillin 1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.

Protocols

Product Specific Protocols
WB protocol for Fibrillin 1 antibody 83836-4-RRDownload protocol
IHC protocol for Fibrillin 1 antibody 83836-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols