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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    WB analysis of L02 using 66158-1-Ig (same clone as 66158-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66158-1-Ig (same clone as 66158-1-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    WB analysis of human peripheral blood platelets using 66158-1-Ig (same clone as 66158-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human liver cancer using 66158-1-Ig (same clone as 66158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human liver cancer using 66158-1-Ig (same clone as 66158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human kidney using 66158-1-Ig (same clone as 66158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IHC staining of human kidney using 66158-1-Ig (same clone as 66158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Fibrinogen Gamma Chain Monoclonal antibody (66158-1-Ig)

    IF Staining of L02 using 66158-1-Ig (same clone as 66158-1-PBS)

    Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

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Fibrinogen Gamma Chain Monoclonal antibody, PBS Only

Fibrinogen Gamma Chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66158-1-PBS
Clone No.2B2B8

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FGG, PRO2061, 2B2B8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66158-1-PBS targets Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Fibrinogen Gamma Chain fusion protein Ag8817 相同性解析による交差性が予測される生物種
Full Name fibrinogen gamma chain
Calculated molecular weight453aa,52 kDa; 437aa,50 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007044
Gene Symbol Fibrinogen Gamma Chain
Gene ID (NCBI) 2266
RRIDAB_2881554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02679
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.