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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fibroblast activation protein alpha Polyclonal antibody

Fibroblast activation protein alpha Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27596-1-AP

Synonyms

FAP, FAPA, EC:3.4.21.26, EC:3.4.21.-, EC:3.4.14.5

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Fibroblast activation protein alpha Polyclonal ant (27596-1-AP)

    IHC staining of human breast cancer using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Fibroblast activation protein alpha Polyclonal ant (27596-1-AP)

    IHC staining of human breast cancer using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Fibroblast activation protein alpha Polyclonal ant (27596-1-AP)

    IHC staining of mouse liver using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Fibroblast activation protein alpha Polyclonal ant (27596-1-AP)

    IHC staining of mouse liver using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman breast cancer tissue, mouse liver
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27596-1-AP targets Fibroblast activation protein alpha in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibroblast activation protein alpha fusion protein Ag26695 相同性解析による交差性が予測される生物種
Full Name fibroblast activation protein, alpha
Calculated molecular weight 760 aa, 88 kDa
GenBank accession numberBC026250
Gene symbol FAP
Gene ID (NCBI) 2191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Fibroblast activation protein alpha antibody 27596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols