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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using Fibromodulin Polyclonal antibody (13281-1-AP)

    WB analysis of human liver using 13281-1-AP (same clone as 13281-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13281-1-AP (Fibromodulin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Fibromodulin Polyclonal antibody (13281-1-AP)

    IF Staining of HepG2 using 13281-1-AP (same clone as 13281-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Fibromodulin antibody (13281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13281-1-PBS in a different storage buffer formulation.

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Fibromodulin Polyclonal antibody, PBS Only

Fibromodulin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13281-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FMOD, Collagen-binding 59 kDa protein, FM, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13281-1-PBS targets Fibromodulin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibromodulin fusion protein Ag4030 相同性解析による交差性が予測される生物種
Full Name fibromodulin
Calculated molecular weight 376 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC035281
Gene Symbol Fibromodulin
Gene ID (NCBI) 2331
RRIDAB_2105531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06828
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).