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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Fibromodulin Monoclonal antibody (60108-1-Ig)

    WB analysis of human kidney using 60108-1-Ig (same clone as 60108-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Fibromodulin Monoclonal antibody (60108-1-Ig)

    WB analysis of human kidney using 60108-1-Ig (same clone as 60108-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using Fibromodulin Monoclonal antibody (60108-1-Ig)

    WB analysis of human stomach using 60108-1-Ig (same clone as 60108-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Fibromodulin Monoclonal antibody (60108-1-Ig)

    IHC staining of human hepatocirrhosis using 60108-1-Ig (same clone as 60108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 60108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Fibromodulin Monoclonal antibody (60108-1-Ig)

    IHC staining of human hepatocirrhosis using 60108-1-Ig (same clone as 60108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 60108-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Fibromodulin Monoclonal antibody, PBS Only

Fibromodulin Monoclonal Antibody for IHC, Indirect ELISA
Cat No. 60108-1-PBS
Clone No.3E9D10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60108-1-PBS targets Fibromodulin in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibromodulin fusion protein Ag4030 相同性解析による交差性が予測される生物種
Full Name fibromodulin
Calculated molecular weight 376 aa, 43 kDa
GenBank accession numberBC035281
Gene Symbol Fibromodulin
Gene ID (NCBI) 2331
RRIDAB_2105538
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ06828
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).